Guruh talabasi ning biorganik kimyo fanidan amaliy mashg’uloti namangan 2023
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- Bu səhifədəki naviqasiya:
- Bioorganik kimyo” umumiy kursidan laboratoriya ishlari
- 1- laboratoriya mashg’uloti Aminokislota va oqsillarga xos sifat reaktsiyalari
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NAMANGAN DAVLAT UNIVERSITETI TABIIY FANLAR FAKULTETI KIMYO YO’NALISHI _________________guruh talabasi ________________________________________________ning BIORGANIK KIMYO fanidan AMALIY MASHG’ULOTI NAMANGAN 2023 “Bioorganik kimyo” umumiy kursidan laboratoriya ishlari
Muammoli-modulli o`qitish texnologiyasini qo`llash bilan laboratoriya mashg`ulotlarini o`tkazish (og`zaki bayon qilish, suhbat, munozara, nazariya bo`yicha kollekvium topshirish, sintezni bajarish). Bu texnologiya asosida talabalarda tanqidiy fikrlashni shakllantirish. Olingan birikmalarning fizik-kimyoviy konstantalarin aniqlash. 1- laboratoriya mashg’uloti Aminokislota va oqsillarga xos sifat reaktsiyalari _____________________ Mavzu. 1 laboratoriya ishi 1-Tajriba. Glycinning Ninhydrin Bilan Reaktion. Tube 4 Tomchi 1% Li Glycin Erythmasi va 2 Tomchi 0,1% Li Ninhydrin Eritmasidan Tomizing. Aralashmani asta-sekin kukimtir-kizil rank hosil bulguncha kizdiring. _______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ __________________________ Mavzu. 2 laboratoriya ishi 2-tajriba. Glitsinning formaldegid bilan reaksiyasi. Probirkaga 5 tomchi 1% li glitsin eritmasidan va 1 tomchi metil kzizil indikatoridan solinadi. Eritma sariq rangga bo‘yaladi (muxit neytral). Olingan aralashmaga teng mikdorda formalin qo‘shiladi va qizil rang xosil bo‘lishiga e’tibor beriladi. Bu reaksiya “formol titrlash” nomi bilan α -aminokislotalarni miqdoriy aniqdash uchun foydalaniladi. Eritma rangining o‘zgarishi nima bilan bog‘liqligi xaqida xulosa chikdring. ______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ _______________________ Mavzu. 3 laboratoriya ishi 3-tajriba. Glitsinning nitrit kislota bilan reaksiyasi. Probirkaga 5 tomchi 1% li glitsin eritmasidan va teng xajmda 5% li natriy nitrit eritmasidan solinadi va 2 tomchi konsentrlangan sirka kislota qo‘shib, aralashma oxista chayqatiladi. Gaz ajralib chiqishi kuzatiladi. Bu reaksiya aminokislotalardagi aminoguruxlarni miqdoriy aniqlash uchun ishlatiladi: _______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Mavzu. 4 laboratoriya ishi 4-tajriba sisteinga rangli reaksiya Probirkaga 5 tomchi 1% li sistein eritmasidan va 2 tomchi 10% li NaOH eritmasidan tomizing. Aralashmani qaynaguncha qizdiring, so‘ng ikki tomchi qo‘rg‘oshin (II) asetatning 10% li eritmasidan tomizing. Probirka tubida qo‘rgoshin sulfidning kul rang qora chukmasi xosil bo‘ladi. Mavzu. 5 laboratoriya ishi 5-tajriba. Peptid bosta biuret reaksiyasi Probirkaga 5—6 tomchi tuxum oqsili eritmasidan soling va teng xajmda NaOH ning 10% li eritmasidan ko‘shing. Probirka devori buylab 1—2 tomchi CuSO4 eritmasidan tomizing. Qizil pushti rang xosil bo‘ladi. Bu reaksiya peptid bog‘ saqlovchi barcha birikmalarga sifat reaksiya xisoblanadi.Peptid bog‘ga biuret reaksiyasi. Kizil pushti rangli xelat kompleks _______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ __________________ Mavzu. 6 laboratoriya ishi 6-tajriba. Oqsillarning ksantoprotein reaksiyasi. Probirkaga 10 tomchi tuxum okosili eritmasidan va 2 tomchi konsentrlangan nitrat kislotadan soling. Aralashmani chayqatishni to‘xtatmasdan asta-sekin qizdiring. Eritma va chukma sariq rangga kiradi. Probirkani sovutib, asta-sekin 1—3 tomchi 10% li NaOH eritmasidan tomizing. Tuq zarg‘aldoq rang xosil bo‘ladi. Bu reaksiya aromatik aminokislotalarga (fenilalanin, tirozin) xos reaksiya bo‘lib, shunday aminokislotalar qoldigani saqdovchi oqsillar xam bu reaksiyani beradi: Yüklə 371,38 Kb. Dostları ilə paylaş:
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