RUAS
Department of Biotechnology (2021-22)
Immunology and Molecular
Biology Laboratory
Experiment 1
OUCHTERLONY DOUBLE DIFFUSION
Introduction
Immunodiffusion in gels encompasses a variety of techniques, which are useful for the
analysis of antigens and antibodies. Gel immunodiffusion can be classified into two groups:
1.
Single Immunodiffusion
2.
Double Immunodiffusion
In the Ouchterlony double diffusion, both the antigen and the antibody diffuse toward each
other in a semisolid medium to a point till their optimum concentration is reached. A band
of precipitation occurs at this point. The qualitative Ouchterlony Test can simultaneously
monitor multiple Antibody-Antigen system and can be used to identify particular antigens in
a preparation. This procedure was developed by Örjan Ouchterlony in 1948.
