Organizing co mmittee

 
 

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Poster presentation 
329 
STUDY OF ANTIARRHYTHMIC ACTIVITY OF MODIFIED 
FORMS OF AMIODARON 
 
V.V. Uzbekov
1
, I.Z. Jumayev
2
, Sh.Yu. Rustamov
2
, Yu.I. Oshchepkova
1*
,
P.B. Usmanov
2
, Sh.I. Salikhov
1
 
 
1) A.S. Sadykov Institute of Bioorganic Chemistry Academy of Sciences of the Republic 
of Uzbekistan, *
e-mail: [e-mail]

2) Institute of Biophysics and Biochemistry National University of Uzbekistan
 
Heart rhythm disturbances are one of the most complex and urgent problems of 
modern cardiology. Arrhythmias are detected both in various cardiovascular diseases 
and in healthy individuals. The spectrum of clinical manifestations of cardiac 
arrhythmias varies from asymptomatic to severe course, from prognostically 
insignificant arrhythmias to determining the nature of the long-term outcome. 
Amiodarone is the most effective of the existing antiarrhythmic drugs. It is even called 
"arrhythmolytic drug". Along with all the advantages, the main disadvantage of 
amiodarone is the likelihood of developing many extracardiac side effects, which are 
observed in 10-52% of patients with long-term use of the drug. 
The aim of this work is to study the antiarrhythmic and inotropic activity of 
complexes of amiodarone hydrochloride (AmdHC) with glycyrrhizic acid (GA) and its 
monoammonium salt (MASGA) in models of CaCl
2
-induced arrhythmia in comparison 
with АmdHC. 
The study in experiments on the papillary muscle of the rat heart was carried out on 
the complexes of GA-AmdHC (2:1), GA-AmdHC (4:1), GA-AmdHC (8:1), MASGA-
AmdHC (2:1), MASGA-AmdHC (4:1) and MASGA-AmdHC (8:1). It was determined 
that in the presence of complexes GA-AmdHC (2:1) (5-100 μM), GA-AmdHC (4:1) (5-
60 μM), GA-AmdHC (8:1) (5-40 µM), MASGA-AmdHC (2:1) (5-120 µM), MASGA-
AmdHC (4:1) (5-100 µM) and MASGA-AmdHC (8:1) (5-30 µM) the papillary muscle 
contraction force decreases to 12.3±5.5%, 16.4±3.9%, 6.2±3.4%, 4.3±3.5%, 3.8±3.5 % 
and 2.7±2.4%, respectively, from the control level. Based on the conducted studies, it 
can be assumed that GA-AmdHC (8:1) and MASGA-AmdHC (8:1) have a stronger 
negative inotropic effect than amiodarone and other complexes. When studying the 
effects of complex compounds on the CaCl
2
-induced arrhythmia model, it was found 
that their addition during the second phase of CaCl
2
action leads to the restoration of 
papillary muscle contractions in response to stimulation. In control experiments with 
amiodarone, it was found that, in its presence, the restoration of muscle contractions 
occurs 10 min after its introduction into the incubation medium. In the presence of GA-
AmdHC (8:1), the restoration of muscle contractions occurred within 7 minutes after its 
introduction into the incubation medium. A more pronounced effect on the background 
of CaCl
2
-induced arrhythmia has MASGA-AmdHC (8:1), in the presence of which the 
restoration of muscle contractions was observed within 3 minutes after its introduction 
into the incubation medium. The data obtained indicate that the modification of the 
structure of AmdHC by the introduction of MASGA provides the complex MASGA-
AmdHC (8:1) with a higher negative inotropic and antiarrhythmic activity. Such an 
increase in the inotropic and antiarrhythmic activity of the MASGA-AmdHC (8:1) 
complex may be due to the presence of additional MASGA groups in its structure, 
which can increase its lipophilicity and, accordingly, membrane activity. 

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Poster presentation 
330