Organizing co mmittee

1
-
3
) were isolated 
from the chloroform fraction of plant.
Investigation by 1D and 2D NMR spectral data of compounds and their comparison 
with the literature as well as direct comparison with authentic samples the isolated 
compounds were identified as 
acacetin
(
1
), 
pectolinarigenin (
2
) and 
scopoletin (
3
). All 
compounds were identified first time from 
A. persica
. 
Acknowledgement
This work was financially supported by the Join Laboratory for Preparation 
Technology and Quality Standards of Natural Medicines Established by the Central 
Asian of Drug Discovery and Development of Chinese Academy of Sciences and 
Institute of the Chemistry of Plant Substances, Academy of Sciences, as well as the 
Alliance of International Science Organizations for Young Talents (ANSO) scholarship.
References 
1.
E.A. Birisibe, U.E. Umoren, F. Brisibe, P.M. Magalhaes, J.F. Ferreira, D. Luthria, X. 
Wu, R.L., 
Food Chem
. 115 (4), 1240-1246 (2009). 
2.
A. Taleghani, S. Ahmad Emami, Z. Tayarani-Najaran, Bioorganic & Medicinal 
Chemistry, 28 (1), 115180 (2020).

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Poster presentation 
105 
STEROID GLYCOSIDE FROM Silene tomentella 
S.S. Narzullaev, U.Yu. Yusupova,
 
D.A. Usmanov, N.Sh. Ramazonov
 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
 
Silene 
is a large genus with more than 700 species growing in various temperate 
regions of the world [1]. Several phytochemical studies have been carried out on 
Silene 
species, highlighting the occurrence of ecdysteroids, triterpene saponins, terpenoids 
[2,3].
However, the same metabolites responsible for increasing plant production 
performance can also make them undesirable for human consumption. Steroid 
glycosides are a class of wide-spread natural products having either terrestrial or marine 
origins [4]. 
Thus, on the basis of the biological activities reported for 
Silene 
species, the 
phytochemical investigation of 
S. tomentella 
was carried out. Herein, we report the 
isolation and structural characterization by NMR experiments of steroid glycoside, 
which investigated for the first time from this plant.
The collected plant material was kept in the dark and air-dried for 10 days. The air-
dried plant material (1 kg) was chopped into thin pieces and extracted with 90% 
aqueous MeOH (4 × 5L, each soaking was continued for 1 week). A methanol extract of 
the roots of 
S. tomentella
was suspended in H
2
O and partitioned with hexane and n-
BuOH. The BuOH-soluble extract (15.7 g) was subjected to CC (SiO
2
(780g); 
AcOEt/MeOH gradient 100:0; 0:100) to yield 18 fractions (Frs. A – R). Fr. H (4.5 g; 
AcOEt/MeOH 4:1) was subjected CC (SiO
2
(200 g); with MeOH/H
2
O 0:100, 10:90, 
30:70, 50:50, 70:30, 100:0) to yield six fractions (Frs. H1 – H6). Fr. H6 (12.2 mg; 
asetonitril/H
2
O 0:100-100:0), which was purified by Sephadex G-15 and yielded one 
individual compound lineolon. 
The structure of this compound was determined using chemical and spectroscopic 
methods, such as 1D and 2D nuclear magnetic resonance (NMR) [heteronuclear single 
quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC)], as 
well as high-resolution electrospray ionisation mass spectrometry (HR-ESI-MS) 
experiments.