www.scientificprogress.uz
Page 408
24. Cai, Y.P., Chen, L., Sun, S., Wu, C.X., Yao, W.W., Jiang, B.J., Han, T.F., Hou,
W.S., 2018b. CRISPR/Cas9-mediated deletion of large genomic fragments in soybean.
Int. J. Mol. Sci. 19, 3835. https://doi.org/10.3390/ijms19123835.
25. Do, P.T., Nguyen, C.X., Bui, H.T., Tran, L.T.N., Stacey, G., Gillman, J.D.,
Zhang, Z.J., Stacey, M.G., 2019. Demonstration of highly efficient
dual gRNA
CRISPR/Cas9 editing of the homeologous GmFAD2-1A and GmFAD2-1B
genes to
yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean. BMC Plant
Biol. 19, 311. https://doi.org/10.1186/s12870-019-1906-8.
26. Bai, M.Y., Yuan, J.H., Kuang, H.Q., Gong, P.P., Li, S.N., Zhang, Z.H., Liu, B.,
Sun, J.F., Yang, M.X., Yang, L., et al., 2019. Generation of a multiplex mutagenesis
population via pooled CRISPR -Cas9 in soya bean. Plant Biotechnol. J. e13239
https://doi.org/ 10.1111/pbi.13239.
27. Ma X, Liu YG. CRISPR/Cas9-Based Multiplex Genome Editing in Monocot and
Dicot Plants. Curr Protoc Mol Biol. 2016 Jul 1;115:31.6.1-31.6.21. doi:
10.1002/cpmb.10.
28.
https://www.idtdna.com/pages/education/decoded/article/annealing-
oligonucleotides