Adabiyotlar: 1. Fillipovich YU.B. i dr. Praktikum po obshey bioximii. M., 1975, 39 s.
2. Kochetkov G.A. Prakticheskoe rukovodstvo po enzimologii. M., 1980, 14 s.
________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________
_______________________________________________________________________________
3-LABARATORIYA ISHI
Nuklein kislotalarga xos sifat reaktsiyalari
__________________________
1-Mavzu: Nuklein kislotalar (RNK, DNK) ni tabiiy manbalar(paxta chigiti, undirilgan mosh, loviya, fasol, no`xat) dan ajratib olish
1-ish
Fenol yordamida deproteinlash orqali paxta chigitidan
DNK ajratib olish
Ishning umumiy izoshi. Oldindan yog`sizlantirilgan paxta chigiti kununidan DNK-proteidni dodetsilsulfat yordamida eritib olinib, spirt bilan cho`kmaga tushiriladi.
Jihozlar va reaktivlar. 100 ml-li o`lchov silindri, shovoncha, sentrifuga, SF-26, 100 ml-li stakanlar, standart tuzi eritma – STE (0,14 M NaCl, 0,05 M Na-tsitrat pH 8,3), suvga to`yitirilgan fenol, 200 ml 65%-li spirt, 10%-li DDS-Na.
Dostları ilə paylaş: |
