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δ-endotoksinlarining ta’siri
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δ-endotoksinlarining ta’siri
№ Variantlar Unib chiqqan nihollar soni, tup Unib chiqqan nihollar, % hisobida Sog‘lom nihollar soni, tup Sog‘lom nihollar, % hisobida Nimjon nihollar soni, tup Nimjon nihollar, % hisobida 1 Nazorat 37 74
31 83,7
6 16,2
2 δ-endotoksinlar suspenziyasi 35
70 32
91,4 3 8,5 3 Fuzarium oxysporium + δ- endotoksinlar suspenziyasi
27 54
23
85,1
4
14,8 4
oxysporium 25
50 19
76 6 24
3.14-jadval. Laboratoriya sharoitida sitaxromoksidaza bilan bog‘liklikda mikroorganizmlarga ta’siri (lunkali usulda) Tajriba mikroorganizmlari Sitoxromoksidaza mavjudligi Lizis bo‘lgan maydon o‘lchami (sm) Neisseria sicca 10 + 0,60 ±0,05 Neisseria flavescens 2 + 0,42 ±0,03 Ervinia carotavora 72 + 0,96 ±0,08 Ervinia carotavora 115 + 0,90 ±0,07 Ervinia carotavora 216 + 0,77±0,08 Corinebacterium insidiosum 14 - - Bacillus polymixa 1532 + 0,30±0,01 Agrobacterium tumefaciens 8628 - - Staphyilococcus saprophyticus 12 - - Escherichia coli 22 - - SHuningdek, unib chiqqan nihollarga nisbatan nimjon (kasallangan) nihollar soni bo‘yicha solishtirilganda ham yuqoridagilarga o‘xshash ma’lumotlar olindi. Olingan natijalar asosida laboratoriya sharoitida ba’zi bir fitopatogen mikroorganizmlarga Bacillus thuringiensis entomopatogen bakteriyasi delta endotoksinlarining ta’sir doirasi kengligi aniqlandi. 86.2% biologik faollikka ega ekanligi aniqlandi. YUqoridagi ma’lumotlarga asoslanib Bacillus thuringiensis bakteriyasi δ- endotoksinlari o‘simliklarni o‘stirish xususiyatini emas, balki sog‘lomligini, kasallik va zararkunandalarga chidamliligini sezilarli darajada oshiradi degan xulosaga kelish mumkin.
3.15-jadval Bakterioz kasallik bilan zararlangan o‘simliklarga (kartoshka: Umid navi) delta- endotoksinning ta’sirini kichik dala sharoitida o‘rganish (kuzatish muddati, 30 kun)
Namunalar Eritma bilan (delta- endotoksin) urug‘ning ishlov
berilishi Tuproqning sun’iy zararlan- tirilishi Sog‘lom
o‘simliklar miqdori
Nobud bo‘lgan
o‘simliklar soni
Umumiy miqdordan nobud bo‘lgan
o‘simliklar, % 1 (nazorat) - - 182±3.82 20.2 ± 3.6 11.5
2 + + 192±2.7 16.4 ± 2.7 8.6 3
+ 162±1.2
43.6± 8.6 27
4 + + 169±4.6 29.7± 6.5 15.3 Takrorlanish n=5 F xaq.
= 86.2 F 05 = 3.12 F xaq. = 185.3 F 05 = 3.14
NSR 05 = 6.9
NSR 05
= 1.5
YUqorida keltirilgan ilmiy manbalarga asoslangan holda laboratoriya sharoitida ba’zi bir fitopatogen mikroorganizmlarga Bacillus thuringiensis entomopatogen bakteriyasi delta endotoksinlarining ta’sir doirasi kengligi, ya’ni 86.2% biologik faollikka ega ekanligi aniqlandi.
45 XULOSA 1. Bacillus thuringiensis bakteriyasi delta endo-toksinlarining o‘simliklar o‘sishiga ta’siri o‘rganilib, o‘sish tezligiga 2.5-3.3% tezlashtirish imkoniyati mavjudligi aniqlandi. Boshoqli o‘simliklar misolida delta endo-toksinlarining tozalangan preparati 9-23% gacha unuvchanlikni oshirishi hamda zamburug‘li kasalliklarga 86.2% gacha fungitsid ta’sir ko‘rsatishi aniqlandi. 2. SHuningdek, ushbu biopreparatning nafaqat antibakterial, fungitsid balkim insektitsid xususiyatlari ko‘rsatib berildi. YUqoridagi ma’lumotlarga asoslanib Bacillus thuringiensis bakteriyasi δ-endotoksinlari o‘simliklarni o‘stirish xususiyatini emas,
balki sog‘lomligini, kasallik va
zararkunandalarga chidamliligini sezilarli darajada oshiradi degan xulosaga kelindi.
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51 Ilova CHOP ETILGAN ILMIY ISHLAR RO‘YXATI
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